Sequences placed on Twist after codon optimization and estimated for cloning via restriction enzymes
Some annotation:
promoter-RBS-hFerritinH- GS linker-SpyCatcher003-6xHis
promoter-RBS-hFerritinH- GS linker-SpyTag002-6xHis
promoter-RBS-SpyCatcher003- GS linker-nanobody (anti GFP)-6xHis
promoter-RBS-SpyCatcher003- GS linker-Zif268 -6xHis (DNA sequence detection)
promoter-RBS-SpyTag002- GS linker-nanobody (anti GFP)-6xHis
promoter-RBS-SpyCatcher003- GS linker-Zif268 -6xHis (DNA sequence detection)
Summary for orders with sequence and full sequence after simulated cloning by restriction on Twist
https://docs.google.com/spreadsheets/d/1x8j__WZBYyHrJyXAFm924BlRe8EUV6NPgQ6bSPMzjqw/edit?usp=sharing